Journal: iScience

Article Title: Neuronal responses to cytokines limit intestinal hypermotility and systemic effects of colonic inflammation

doi: 10.1016/j.isci.2026.116291

Figure Lengend Snippet: Responses to IFNγ and IL-10 by Nav1.8-Cre + neurons limits GI motility (A–D) Total GI transit in Nav1.8-Cre mice crossed with (A) Il4ra fl/fl ( N = 8 Il4ra fl/fl , 9 Il4ra ΔNav1.8 , expt. = 2); (B) Ifnar1 fl/fl ( N = 6 Ifnar1 fl/fl , 7 Ifnar1 ΔNav1.8 , expt. = 2); (C) Ifngr1 fl/fl ( N = 9 Ifngr1 fl/fl , 12 Ifngr1 ΔNav1.8 , expt. = 2); or (D) Il10ra fl/fl ( N = 10 Il10ra fl/fl , 8 Il10ra ΔNav1.8 , expt. = 3). (E–I) Additional analyses of Nav1.8-Cre crossed to Ifngr1 fl/fl (top) or Il10ra fl/f (bottom) mice. (E) Body weight ( N = 4 Ifngr1 fl/fl , 10 Ifngr1 ΔNav1.8 ; 6 Il10ra fl/fl , 9 Il10ra ΔNav1.8 , expt. = 2). (F) Immune cell phenotype in the colon ( N = 5 Ifngr1 fl/fl , 6 Ifngr1 ΔNav1.8 ; 6 Il10ra fl/fl , 6 Il10ra ΔNav1.8 , expt = 2). (G) Representative histology of the proximal colon ( N = 4 each). Scale bars represent 400 μm. (H) Non-metric Multidimensional Scaling plots of Bray-Curtis dissimilarity of 16 S rRNA sequencing of the terminal fecal pellet ( N = 9 Ifngr1 fl/fl , 9 Ifngr1 ΔNav1.8 ; 7 Il10ra fl/fl , 9 Il10ra ΔNav1.8 ). (I) Fecal water weight ( N = 7 Ifngr1 fl/fl , 12 Ifngr1 ΔNav1.8 ; 10 Il10ra fl/fl , 7 Il10ra ΔNav1.8 , expt. = 2 each). Mice were 7- to 10-week old or as indicated. Each dot represents an individual mouse. N is the total number of mice. Significance was determined by unpaired two-tailed Student’s t test. Data are presented as mean ± SEM. ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001. ns, not significant. See also and .

Article Snippet: Ifngr1 −/− , Jackson Laboratory , .

Techniques: Sequencing, Two Tailed Test

Journal: iScience

Article Title: Neuronal responses to cytokines limit intestinal hypermotility and systemic effects of colonic inflammation

doi: 10.1016/j.isci.2026.116291

Figure Lengend Snippet: Responses to cytokines by Nav1.8-Cre expressing neurons limit the severity of intestinal injury Ten-week-old Nav1.8-Cre mice bred to Jak1 fl/fl , Ifngr1 fl/fl , or Il10ra fl/fl were given 2% DSS in drinking water for 7 days. (A) Body weight as percentage of starting weight ( N = 9 Jak1 fl/fl , 12 Jak1 ΔNav1.8 , expt. = 3; 7 Ifngr1 fl/fl , 7 Ifngr1 ΔNav1.8 , expt. = 2; 14 Il10ra fl/fl , 12 Il10ra ΔNav1.8 , expt. = 3). (B) Total GI transit rate on day 10 after start of DSS ( N = 4 Jak1 fl/fl , 4 Jak1 ΔNav1.8 ; 5 Ifngr1 fl/fl , 5 Ifngr1 ΔNav1.8 ; 5 Il10ra fl/fl , 4 Il10ra ΔNav1.8 , expt. = 2). (C) Colon length on day 10 after start of DSS ( N = 4 Jak1 fl/fl , 5 Jak1 ΔNav1.8 ; 5 Ifngr1 fl/fl , 4 Ifngr1 ΔNav1.8 ; 6 Il10ra fl/fl , 6 Il10ra ΔNav1.8 ). (D) Lcn2 of terminal fecal pellet on day 8 after start of DSS ( N = 7 Jak1 fl/fl , 7 Jak1 ΔNav1.8 ; 7 Ifngr1 fl/fl , 7 Ifngr1 ΔNav1.8 ; 6 Il10ra fl/fl , 5 Il10ra ΔNav1.8 , expt. = 2 each). (E) Histologic analysis of the proximal colon on day 10 after DSS. (Left) Summary of inflammation scores (range 0–3, see Method details; N = 4 Jak1 fl/fl , 4 Jak1 ΔNav1.8 ; 5 Ifngr1 fl/fl , 5 Ifngr1 ΔNav1.8 ; 5 Il10ra fl/fl , 4 Il10ra ΔNav1.8 , expt. = 2). Statistics were performed on combined Nav1.8-Cre – vs. Cre + data. (Right) Representative histology of the tissues from colitis scores = 0 Jak1 fl/fl , 1 Jak1 ΔNav1.8 ; 1 Ifngr1 fl/fl , 2 Ifngr1 ΔNav1.8 ; 0 Il10ra fl/fl , 2 Il10ra ΔNav1.8 . Arrows indicate areas of inflammation (score 1, neutrophils in lamina propria; score 2, plasma cells and lymphocytes in submucosa). Scale bars represent 50 μm. Each dot represents an individual mouse. N is the total number of mice. Significance was determined by two-way ANOVA (A) or unpaired two-tailed Student’s t test. Data are presented as mean ± SEM. ∗ p < 0.05. ns, not significant. See also .

Article Snippet: Ifngr1 −/− , Jackson Laboratory , .

Techniques: Expressing, Clinical Proteomics, Two Tailed Test

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Essential role of MHC II in the antitubercular efficacy of pyrazinamide

doi: 10.1128/aac.01264-25

Figure Lengend Snippet: PZA activity is dependent on the oxidative burst. PZA bactericidal activity in Mtb strain H37Rv infected ( A ) C57BL/6J, ( B ) ifngR1 KO, ( C ) C57BL/6NJ, and ( D ) p47 phox KO BMDMs. Infected resting and IFN-γ activated BMDMs were untreated or treated with PZA (200 µg/mL or 400 µg/mL). Cultures were plated for bacterial enumeration at indicated timepoints. Statistical significance was calculated based on multiple comparisons with two-way ANOVA with Bonferroni correction. ** P < 0.005 .

Article Snippet: WT C57BL/6J (#000664), WT C57BL/6NJ (#005304), B6N.129S2- Ncf1 tm1Shl /J (PHOX47 phox−/− , #027331), B6129S7- IfngR1 tm1Agt /J (IFNGR1 −/− , #003288), and B6.129S2- H2 dlAb1-Ea /J (MHC II −/− , #003584) were purchased from the Jackson Laboratory.

Techniques: Activity Assay, Infection

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Essential role of MHC II in the antitubercular efficacy of pyrazinamide

doi: 10.1128/aac.01264-25

Figure Lengend Snippet: PZA failure in ifngR1 KO and MHC II KO mice. Mtb strain H37Rv total bacterial burden in lungs, livers, and spleens of C57BL/6J, ifngR1 KO , and MHC II KO mice. All mice were infected with low-dose aerosol Mtb , and the infection was allowed to progress for 3 weeks in order for cell-mediated immunity to initiate (pretreatment). Mice were subsequently treated daily for 5 days a week for 2 weeks with either vehicle control (sterile water), PZA (150 mg/kg), and INH (30 mg/kg). Upon euthanasia, organs were removed, homogenized, and plated for bacterial enumeration at indicated timepoints. Empty circles on the x -axis indicate animals for which no bacterial growth was detected. Statistical significance was calculated based on paired two-tailed t -tests, and P values were adjusted for multiple comparisons. *P < 0.05, ** P < 0.05, *** P < 0.0005. ns, not significant.

Article Snippet: WT C57BL/6J (#000664), WT C57BL/6NJ (#005304), B6N.129S2- Ncf1 tm1Shl /J (PHOX47 phox−/− , #027331), B6129S7- IfngR1 tm1Agt /J (IFNGR1 −/− , #003288), and B6.129S2- H2 dlAb1-Ea /J (MHC II −/− , #003584) were purchased from the Jackson Laboratory.

Techniques: Infection, Aerosol, Control, Sterility, Two Tailed Test

Journal: bioRxiv

Article Title: Strong sustained type I IFN signaling acts cell intrinsically to impair IFNγ responses and cause tuberculosis susceptibility

doi: 10.64898/2026.01.19.700487

Figure Lengend Snippet: (A) Representative flow cytometry plots of a Mtb -mWasabi infected B6 mouse gated on live CD45+ B220-CD90.2-cells to identify interstitial macrophages (CD64+ MerTK high SigleF-), which can be grouped into bystander (mWasabi-) and infected (mWasabi+) cells. (B) MFI of CXCL9 and (C) NOS2 staining in IMs from Mtb -infected B6 and Ifngr1 −/− mice at day 25 pi grouped in bystander and infected IMs. (D) MFI of NOS2 staining in IMs from Mtb -infected B6 and Sp140 −/− mice at day 25 pi. (E) MFI of NOS2 staining in IMs from Mtb -infected Sp140 −/− , Sp140 −/− Ifnar1 −/− , Sp140 −/− Irf9 −/− and Sp140 −/− Stat2 −/− mice at day 28-29 pi. (F) MFI of NOS2 staining in IMs from Mtb -infected B6 and B6. Ifnar1 −/− mice at day 25-26 pi. Statistical significance was calculated in (B, C, E) with Brown-Forsythe and Welch ANOVA test and Dunnett’s T3 multiple comparisons test, in (D, F) with Welch’s t-test. *p < 0.05, **p < 0.01, ***p < 0.001, ns = not significant.

Article Snippet: Mouse lines used are C57BL/6J (B6), B6.129S2- Ifnar1 tm1Agt/ Mmjax ( Ifnar1 −/− ), B6.129S7- Ifngr1 tm1Agt/J ( Ifngr1 −/− ), C57BL/6J- Ptprc em6Lutzy/J (CD45.1) and B6.Cg- Mx1 tm1.1Agsa/J ( Mx1-g fp ) that were purchased from Jackson Laboratories.

Techniques: Flow Cytometry, Infection, Staining

Journal: bioRxiv

Article Title: Strong sustained type I IFN signaling acts cell intrinsically to impair IFNγ responses and cause tuberculosis susceptibility

doi: 10.64898/2026.01.19.700487

Figure Lengend Snippet: (A) Mean Fluorescence Intensity (MFI) of CXCL9 staining in interstitial macrophages (IMs) from Mtb -infected B6 and Ifngr1 −/− mice at day 25 post infection (pi). (B) Colony Forming Units (CFUs) per lung (left panel) and MFI of CXCL9 staining in IMs (right panel) from Mtb -infected B6 and type I IFN susceptible Sp140 −/− mice at day 25 pi. (C) CFUs per lung and (D) MFI of CXCL9 staining in IMs from Mtb -infected Sp140 −/− , Sp140 −/− Ifnar1 –/– , Sp140 −/− Irf9 −/− and Sp140 −/− Stat2 −/− mice at day 28-29 pi. (E) CFUs per lung (left panel) and MFI of CXCL9 staining in IMs (right panel) from Mtb -infected B6 and B6. Ifnar1 −/− mice at day 25-26 pi. Statistical significance was calculated for CFU data in (B, E) with Mann-Whitney test and in (C) Kruskal-Wallis test with Dunn’s multiple comparison test, for MFI data in (A,B,E) Welch’s t-test and in (D) Brown-Forsythe/Welch ANOVA test, both with Dunnett’s T3 multiple comparisons test. *p < 0.05, **p < 0.01, ***p < 0.001, ns = not significant.

Article Snippet: Mouse lines used are C57BL/6J (B6), B6.129S2- Ifnar1 tm1Agt/ Mmjax ( Ifnar1 −/− ), B6.129S7- Ifngr1 tm1Agt/J ( Ifngr1 −/− ), C57BL/6J- Ptprc em6Lutzy/J (CD45.1) and B6.Cg- Mx1 tm1.1Agsa/J ( Mx1-g fp ) that were purchased from Jackson Laboratories.

Techniques: Fluorescence, Staining, Infection, MANN-WHITNEY, Comparison